Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries
نویسندگان
چکیده
منابع مشابه
Continuous affinity-based selection: rapid screening and simultaneous amplification of bacterial surface-display libraries.
A new method for continuous biopanning has been developed. We have combined the power of affinity chromatography with the fecundity of bacteria in a unique process that mimics clonal selection. Mixed populations of bacteria were applied to a fermenter containing the immobilized ligand of interest. Bacteria retained in this affinity fermenter were allowed to grow under continuous washout conditi...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 2001
ISSN: 0264-6021
DOI: 10.1042/0264-6021:3570779